Ancestry, diversity, and genetics of health-related traits in African-derived communities (quilombos) from Brazil.

Brazilian quilombos are communities formed by enslaved Africans and their descendants all over the country during slavery and shortly after its abolition. Quilombos harbor a great fraction of the largely unknown genetic diversity of the African diaspora in Brazil. Thus, genetic studies in quilombos have the potential to provide important insights not only into the African roots of the Brazilian population but also into the genetic bases of complex traits and human adaptation to diverse environments. This review summarizes the main results of genetic studies performed on quilombos so far. Here, we analyzed the patterns of African, Amerindian, European, and subcontinental ancestry (within Africa) of quilombos from the five different geographic regions of Brazil. In addition, uniparental markers (from the mtDNA and the Y chromosome) studies are analyzed together to reveal demographic processes and sex-biased admixture that occurred during the formation of these unique populations. Lastly, the prevalence of known malaria-adaptive African mutations and other African-specific variants discovered in quilombos, as well as the genetic bases of health-related traits, are discussed here, together with their implication for the health of populations of African descent.

Uniparental markers reveal new insights on subcontinental ancestry and sex-biased admixture in Brazil.

The Brazilian population is a product of asymmetric admixture among European men and Amerindian and African women. However, Brazilian subcontinental ancestry is scarcely documented, especially regarding its African roots. Here, we aimed to unveil the uniparental continental and subcontinental contributions from distinct Brazilian regions, including South (n = 43), Southeast (n = 71), the poorly genetically characterized Central-Western region (n = 323), and a subset of unique Brazilian Amerindians (n = 24), in the context of their genome-wide ancestral contributions. The overwhelming majority of European Y haplogroups (85%) contrast sharply with the predominant African and Amerindian mtDNA haplogroups (73.2%) in admixed populations, whereas in Amerindians, non-Native haplogroups could only be detected through the paternal line. Our in-depth investigation of uniparental markers showed signals of an Andean and Central-Brazilian Amerindian maternal contribution to Southeastern and Central-Western Brazil (83.1 ± 2.1% and 56.9 ± 0.2%, respectively), the last having the highest paternal Amerindian ancestry yet described for an admixed Brazilian region (9.7%) and contrasting with higher Southern-Brazilian Amerindian contribution to Southern Brazil (59.6 ± 1%). Unlike the higher African Bantu contribution previously reported for the South and Southeast, a relevant Western African non-Bantu contribution was detected in those regions (85.7 ± 5% and 71.8 ± 10.8% respectively). In contrast, a higher Bantu contribution was described for the first time in the Central-West (64.8 ± 1.3% maternal and 86.9 ± 9.6% paternal). We observed sex-biased signatures consistent with the historically recorded Brazilian colonization and added new insights in the subcontinental maternal ancestry of Brazilians from regions never studied at this level.

Constructed wetlands as an alternative for arsenic removal from reverse osmosis effluent.

In Argentina, drinking water for c.a. 10% of the population has arsenic (As) concentrations higher than those recommended by WHO (10 µg L-1). Reverse osmosis (RO) appears as an immediate and effective solution for As remediation. However, this process has a residual flow known as "rejection" or "concentrate" where dissolved species are more concentrated than in the feed flow. In this study, phytoremediation with subsurface horizontal-flow constructed wetlands (CW) was proposed to reduce As concentration in the RO residues. Experiments were carried out during 419 days at room temperature and using a continuous regime (flow of 36 L d-1, As concentration around 85 µg L-1) of RO rejection from a water treatment plant located in Buenos Aires province, Argentina. The study was performed using prototypes planted with Cyperus haspan (PA), Juncus effusus (PB) and a mix of inert gravel and laterite (substrate) that was used as a control (PC). Results showed that after a stabilization time, As removal (%) was between 30% and 80% in the CW planted with J. effusus and between 10 and 40% with C. haspan. As concentration along CW showed similarities between the prototypes PC and PA. The cumulative mass of As was 62%, 34% and 27% for PA, PB and PC, respectively. The contribution of C. haspan and J. effusus during the experimental time was between 12 and 67% and 22 to 87%, respectively. The bioaccumulation and translocation factors indicated that for J. effusus the accumulation is more important than the translocation process (1.6 and 0.2, respectively), while for C. haspan both factors were similar (1.1 and 1.0, respectively). Results suggested that this technology has the potential for an efficient and environmentally sustainable alternative to RO rejection treatment and disposal regarding As concentration.

Non-destructive methodologies applied to track the occurrence of natural micropollutants in watering: Glycine max as a biomonitor.

Groundwater is habitually used for watering purposes in rural areas where the rainfall is not enough to adequately cover the crop requirements. However, groundwater sources could be naturally contaminated with trace micropollutants like As and associated elements (B, V and F) adversely affecting the plant health. In this work, non-destructive methodologies based on reflectance and chlorophyll emission processes were applied to assess the presence of micropollutants in watering by using a widespread crop (soybean plant). One of the most substantial results is that the co-occurrence of As, V, B and F in the watering solution clearly produced a synergistic effect in the plants. In fact, both reflectance and fluorescence techniques were proved in this work to be effective in detecting non-destructively stress by multielement treatment. Particularly, for reflectance measurements the most sensitive parameters were the derivative peak area between 480 and 560 nm and the chlorophyll content. Furthermore, it was demonstrated that it is possible to successfully use a portable hyperspectral spectroradiometer instead of a conventional spectrophotometer as the determinations performed with both instruments were positively correlated. Concerning fluorescence, variable emission of chlorophyll-a was more sensitive to stress than steady-state emission. The parameter Fv/F0 was a valuable indicator of stress but the quantum yields of PSII and NPQ stood out as the most sensitive indices with variations of around 60 and 100% respectively.

Trace metals and oxidative status in soft tissues of caged mussels (Aulacomya atra) on the North Patagonian coastline.

This study investigated metal accumulation and oxidative effects in mantle, gill and digestive gland of the ribbed mussel Aulacomya atra from the Argentinean North Patagonian coastline. Mussels were transplanted over an 18-month period from a site with low anthropogenic impact to a harbor site with higher seawater concentration of aluminum, chromium, copper, manganese, nickel and zinc. Total trace metal concentration in seawater did not change throughout the 18-month transplant in either site. A. atra bioaccumulated metals in digestive gland, gills and mantle at different levels. Digestive gland had the highest concentration of metals, especially towards the end of the transplant experiment in the harbor area. Mussels transplanted to the harbor site experienced an upregulation in their antioxidant system, which likely explains the lack of oxidative damage to lipids despite higher metal accumulation. These results demonstrate that A. atra selectively accumulates metals from the water column and their prooxidant effects depend on the tissue antioxidant defenses and the exposure time.